​Bioprinting of complex multicellular organs with advanced functionality—recent progress and challenges ahead
LE Bertassoni
Advanced Materials 34 (3), 2101321

​Matrix stiffness regulates lipid nanoparticle-mRNA delivery in cell-laden hydrogels
A Athirasala, S Patel, PP Menezes, J Kim, A Tahayeri, G Sahay, ...
Nanomedicine 42, 10255012022

3D printing of Microgel-loaded Modular Microcages as Instructive Scaffolds for Tissue Engineering,
​Ramesh Subbiah,  ​Christina Hipfinger,  Anthony Tahayeri, Avathamsa Athirasala, Sivaporn Horsophonphong, Greeshma Thrivikraman, Cristiane Miranda Franca, Diana Araujo Cunha, Amin Mansoorifar, Albena Zahariev, James M Jones, Paul G Coelho, Lukasz Witek, Hua Xie, Robert E Guldberg, Luiz E. Bertassoni,  
​Advanced Materials 2020. https://doi.org/10.1002/adma.202001736

​Thrivikraman G, Athirasala A, Gordon R, Zhang L, Bergan R, Keene DR, Jones JM, Xie H, Ferracane JL, Wingender B, Gower L, BertassoniLE. Rapid fabrication of vascularized and innervated cell-laden bone models with biomimetic intrafibrillar collagen mineralization.  Nature Communications. 2019 6;10(1):3520

Bone‐on‐a‐Chip: Microfluidic Technologies and Microphysiologic Models of Bone Tissue
Amin Mansoorifar, Ryan Gordon, Raymond C. Bergan, Luiz E. Bertassoni, 
Advanced Functional Materials, 2020, https://doi.org/10.1002/adfm.202006796
Engineering of an osteoinductive and growth factor‐free injectable bone‐like microgel for bone regeneration
R Subbiah, EY Lin, A Athirasala, GE Romanowicz, ASP Lin, JV Califano, ...
Advanced Healthcare Materials, 22009762023

Opportunities and challenges to engineer 3D models of tumor-adaptive immune interactions
RM Visalakshan, MK Lowrey, MGC Sousa, HR Helms, A Samiea, ...
Frontiers in Immunology 142023

3D Bioprinting of Blood Vessels and Vascular Networks: Progress and Challenges Toward Biofabrication of Functional Vascularized Tissues and Organs
SP Parthiban, P Jurney, A Athirasala, CM França, A Tahayeri, ...
EMERGING TECHNOLOGIES IN BIOPHYSICAL SCIENCES: A WORLD SCIENTIFIC REFERENCE …2023

Oral mucosa equivalents, prevascularization approaches, and potential applications
DS Masson-Meyers, LE Bertassoni, L Tayebi
Connective Tissue Research 63 (5), 514-52952022

Challenges and Perspectives on the Use of Pericytes in Tissue Engineering
GCY Hsu, A Lu, L Bertassoni, CM Franca
Current Tissue Microenvironment Reports 3 (3), 21-352022

Biological aspects in controlling angiogenesis: current progress
M Akbarian, LE Bertassoni, L Tayebi
Cellular and Molecular Life Sciences 79 (7), 34942022

Self-assembly peptide P11-4 induces mineralization and cell-migration of odontoblast-like cells
IJ de Souza Araújo, GN Guimarães, RA Machado, LE Bertassoni, ...
Journal of Dentistry 121, 10411122022

Vascular inflammation exposes perivascular cells to SARS-CoV-2 infection
CM Franca, A Mansoorifar, A Athirasala, R Subbiah, A Tahayeri, ...
bioRxiv, 2022.04. 05.4872402022

3D-printed microgels supplemented with dentin matrix molecules as a novel biomaterial for direct pulp capping
D Cunha, N Souza, M Moreira, N Rodrigues, P Silva, C Franca, ...
Clinical oral investigations, 1-1122022

Advances in Biomaterials for Oral Health
JL Ferracane, CS Pfeifer, LE Bertassoni
Dental Clinics 66 (4), xiii-xiv2022
​​Rodrigues NS, França CM, Tahayeri A, Ren Z, Saboia VPA, Smith AJ, Ferracane JL, Koo H, Bertassoni LE. Biomaterial and Biofilm Interactions with the Pulp-Dentin Complex-on-a-Chip. J Dent Res. 2021 May 26:220345211016429. doi: 10.1177/00220345211016429. ​
Twohig C, Helsinga M, Mansoorifar A, Athirasala A, Tahayeri A, França CM, Pajares SA, Abdelmoniem R, Scherrer S, Durual S, Ferracane J, Bertassoni LE. A dual-ink 3D printing strategy to engineer pre-vascularized bone scaffolds in-vitro. Mater Sci Eng C Mater Biol Appl. 2021 Apr;123:111976. doi: 10.1016/j.msec.2021.111976.
Subbiah R, Thrivikraman G, Parthiban SP, Jones JM, Athirasala A, Xie H, Bertassoni LE. Prevascularized hydrogels with mature vascular networks promote the regeneration of critical-size calvarial bone defects in vivo. J Tissue Eng Regen Med. 2021 Mar;15(3):219-231. doi: 10.1002/term.3166.
Ramesh Subbiah, Marissa A. Ruehle, Brett S. Klosterhoff, Angela S. P. Lin, Marian H. Hettiaratchi, Nick J. Willett, Luiz E. Bertassoni, Andrés J. García, Robert E. Guldberg, Triple Growth Factor Delivery Promotes Functional Bone Regeneration Following Composite Musculoskeletal Trauma, Acta Biomaterialia 2021, ​https://doi.org/10.1016/j.actbio.2021.03.066
SP Parthiban, W He, N Monteiro, A Athirasala, CM França, LE Bertassoni, Engineering pericyte-supported microvascular capillaries in cell-laden hydrogels using stem cells from the bone marrow, dental pulp and dental apical papilla, Scientific Reports 2020, ​https://doi.org/10.1038/s41598-020-78176-7
Maria Elisa Quezado Lima-Verde, S Prakash Parthiban, Antonio Ernando Carlos Ferreira Junior, Paulo Goberlânio de Barros Silva, Edmar Maciel Lima Junior, Manoel Odorico de Moraes, Vicente de Paulo Aragão Sabóia, Luiz E Bertassoni, Ana Paula Negreiros Nunes Alves, Nile Tilapia Fish Skin, Scales, and Spine as Naturally Derived Biomaterials for Tissue Regeneration, Current Oral Health Reports, 2020, https://doi.org/10.1007/s40496-020-00286-y
 Sivaporn Horsophonphong, Ashley Sercia, Cristiane M.França, Anthony Tahayeri, Ashok P. Reddy, Phillip A. Wilmarth, Rudee Surarit, Anthony J. Smithe, Jack L.Ferracane, Luiz E. Bertassoni, Equivalence of human and bovine dentin matrix molecules for dental pulp regeneration: proteomic analysis and biological function, Archives of Oral Biology, 2020, https://doi.org/10.1016/j.archoralbio.2020.104888
Amin Mansoorifar, Ramesh Subbiah, Gabriela de Souza Balbinot, Selvakumar Prakash Parthiban, Luiz E Bertassoni, Embedding cells within nanoscale, rapidly mineralizing hydrogels: A new paradigm to engineer cell-laden bone-like tissue, Journal of Structural Biology, 2020, https://doi.org/10.1016/j.jsb.2020.107636

Luiz E. Bertassoni, Progress and Challenges in Microengineering the Dental Pulp Vascular Microenvironment, Journal of Endodontics 2020,  https://doi.org/10.1016/j.joen.2020.06.033
Yubingqing Qi, Ting Zou, Waruna Lakmal Dissanayaka, Hai Ming Wong, Luiz E Bertassoni, Chengfei Zhang, Fabrication of Tapered Fluidic Microchannels Conducive to Angiogenic Sprouting within Gelatin Methacryloyl Hydrogels, Journal Journal of Endodontics 2021, https://doi.org/10.1016/j.joen.2020.08.026
Ramesh Subbiah, Albert Cheng, Marissa A. Ruehle,  Marian H. Hettiaratchi,  Luiz E. Bertassoni,  Robert E. Guldberg, Effects of Controlled Dual Growth Factor Delivery on Bone Regeneration Following Composite Bone-Muscle Injury, Acta Biomaterialia 2020,   https://doi.org/10.1016/j.actbio.2020.07.026
Amin Mansoorifar, Anthony Tahayeri, and Luiz E. Bertassoni, Bioinspired reconfiguration of 3D printed microfluidic hydrogels via automated manipulation of magnetic inks, Lab on a chip 2020 In press (DOI: 10.1039/d0lc00280a)
Prakash Parthiban S., Avathamsa Athirasala, Anthony Tahayeri, Reyan Abdelmoniem, Anne George, Luiz E. Bertassoni, BoneMA - Synthesis and Characterization of a Methacrylated Bone-derived Hydrogel for Bioprinting of Vascularized Tissues, Biofabrication 2020 In Press (https://doi.org/10.1088/1758-5090/abb11f).
França CM, Tahayery A, Rodrigues, NS, Ferdosiam S, Pupin-Rontani R, Sereda G, Ferracane JL, Bertassoni LE. The tooth on-a-chip: a microphysiologic model system mimicking the biologic interface of the tooth with biomaterials. Lab on a chip 2020 In press (doi: 10.1039/C9LC00915A)
Ha M, Athirasala A, Tahayeri A, Menezes P, Bertassoni LE. Micropatterned Hydrogels and Cell Alignment Enhance the Odontogenic Potential of Stem Cells from Apical Papilla. Dental Materials 2020 36, (1) 88-96
Parthiban SP, Jurney P, Athirasala A, França CM, Tahayeri A, Menezes P, Bertassoni LE. 3D Bioprinting of Blood Vessels and Vascular Networks: Progress and Challenges Toward Biofabrication of Functional Vascularized Tissues and Organs. In Emerging Technologies for Biofabrication and Biomanufacturing edited by Utkan Demirci, Rami El Assal and Pu Chen. 2019 Publisher: World Scientific Publishing 
França CM, Riggers R, Muschler JL, Widbiller M, Lococo PM, Diogenes A,  and Bertassoni LE. 3D-Imaging of Whole Neuronal and Vascular Networks of the Human Dental Pulp via CLARITY and light sheet microscopy. 2019 Scientific Reports 26;9(1):10860.
Bertassoni LE. 3D printing of restorative dental composites and ceramics – toward the next frontier in restorative dentistry. Journal of the Californian Dental Association 2019 47;10 645-651 (Invited review)

França CM, Sercia A, Parthiban SP, Bertassoni LE. Current and Future Applications of 3D Bioprinting in Endodontic Regeneration. Journal of the Californian Dental Association  2019 47;10 653-665 (Invited review)
G.G. Trindade, G. Thrivikraman, PP Menezes, CM França, BS Lima, YMBG Carvalho, E. Souza, MC Duarte, S Shanmugam, LJ Quintans-Júnior, DP Bezerra, LE Bertassoni*, AAS Araújo*. Carvacrol/β-cyclodextrin inclusion complex inhibits cell proliferation andmigration of prostate cancer cells. 2019 Food and Chemical Toxicology. (125) 198-209 (*co-corresponding author)
 França CM, Tahayery A, Rodrigues, NS, Ferdosiam S, Pupin-Rontani R, Ferracane JL Bertassoni LE. The tooth on-a-chip: a microphysiologic model system mimicking the pulp-dentin interface and its interaction with biomaterials. BioRxiv. 2019
França CM, Thrivikraman G, Athirasala A, Tahayeri A, Gower LB, Bertassoni LE. The influence of osteopontin-guided collagen intrafibrillar mineralization on pericyte differentiation and vascularization of engineered bone scaffolds. Journal of Biomedical Materials Research B - Applied Biomaterials. 2018 Sep 29. doi: 10.1002/jbm.b.34244. [In press] 
​Nelson Monteiro, Wenting He, Cristiane Miranda Franca, Avathamsa Athirasala, and Luiz E. Bertassoni. Engineering Microvascular Networks in LED Light-Cured Cell-Laden Hydrogels.  ACS Biomaterials  Science and Engineering. 2018, 4 (7), pp 2563–2570 
Patel S, Athirasala A, Menezes PP, Ashwanikumar N, Zou T, Sahay G, Bertassoni LE. Messenger RNA Delivery for Tissue Engineering and Regenerative Medicine Applications.  Tissue Engineering - Part A. 2018 Jun 7. doi: 10.1089/ten.TEA.2017.0444
Athirasala A, Tahayeri A, Thrivikraman G, Miranda Franca C, Monteiro N, Tran V, Ferracane J, Bertassoni L.  A Dentin-derived hydrogel bioink for 3D printing of cell laden scaffolds for regenerative dentistry. 2017 Nov 17. doi: 10.1088/1758-5090/aa9b4e. Biofabrication.
3D printed versus conventionally cured provisional crown and bridge dental materials. Tahayeri A, Morgan M, Fugolin AP, Bompolaki D, Athirasala A, Pfeifer CS, Ferracane JL, Bertassoni LE. 2017 Oct 27. pii: S0109-5641(17)30447-5. doi: 10.1016/j.dental.2017.10.003. Dental Materials 
​Photopolymerization of cell-laden gelatin methacryloyl hydrogels using a dental curing light for regenerative dentistry. Dental Materials (In-press) 
Athirasala A,  Lins F,  Tahayeri A,  Hinds M,  Smith AJ,  Sedgley C,  Ferracane J,  Bertassoni LE. A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs.  7: 3323. 2017. Scientific Reports

Bertassoni LE. Dentin on The Nanoscale: Hierarchical Organization, Mechanical Behavior and Bioinspired Engineering. 33:637-649. 2017. Dental Materials

Thrivikraman G,  França CM, Athirasala A,  Tahayeri A, Bertassoni LE. Nanomaterials in 3D Bioprinting - Current Approaches and Future Possibilities. In Nanotechnologies in Preventive and Regenerative Medicine. Editor: Vuk Uskokovic Publisher: Elsevier . 2017 
Thrivikraman G,  Athirasala A, Twohig C,  Boda SK, Bertassoni LE.  Biomaterials for Craniofacial Bone Regeneration. ​​Dental Clinics of North America. 2017 
Ferracane J, Bertassoni LE, Pfeifer C (Editors). Biomaterials for Oral Health. Dental Clinics of North America - October 2017
Pereira MA, Santos-Junior RB, Tavares JA, Oliveira AH, Leal PC, Takeshite M, Barbosa-Junior M, Bertassoni LE, Faria-e-Silva AL. No additional benefit of using a calcium hydroxide liner during step-wise caries removal. A randomized clinical trial. Journal of the American Dental Association. 2017 (Accepted)
Bertassoni LE & Swain MV. Removal of Dentin Non-collagenous Structures Results in the Unraveling of Microfibril Bundles in Collagen Type I. Connective Tissue Res.  2016 Sep 22:1-10.
Book: Bertassoni, LE and Coelho, PG. “Engineering load bearing and mineralized tissues”. Series: Advances in Experimental Medicine and Biology. Publisher: Springer  - In-press doi: 10.1007/978-3-319-22345-2
Stankoska K, Sarram L, Smith S, Bedran-Russo AK, Little CB, Swain MV, Bertassoni LE. Immunolocalization and distribution of proteoglycans in carious dentine. Aust Dent J. 2016 Oct 5. doi: 10.1111/adj.12376.
Bertassoni LE, Kury M, Rathsam C, Little CB, Swain MV. The Role of Proteoglycans in the Nanoindentation Creep Behavior of Human Dentin. Journal of Mechanical Behavior of Biomedical Materials
Barabaschi G, Manoharan V, Li Q, Bertassoni LE. Engineering Pre-vascularized Scaffolds for Bone Regeneration. In ‘Engineering Load-bearing and mineralized tissues’; Series: Advances in Experimental Medicine and Biology; Publisher: Springer 2015. In-press doi: 10.1007/978-3-319-22345-2
Obregon F, Vaquette C, Ivanovski S, Hutmacher D, Bertassoni LE. 3D Bioprinting for Regenerative Dentistry and Craniofacial Tissue Engineering. Journal of Dental Research. 2015
Elfallah HM, Bertassoni LE, Charadram N, Rathsam C, Swain MV. Effect of tooth bleaching agents on protein content and mechanical properties of dental enamel. Acta Biomater. 2015 doi:10.1016/j.actbio.2015.03.035 
Thomé T; Erhardt MCG; Ariene L; Bakri IA; Bedran-Russo AK; Bertassoni LE. Emerging polymers in dentistry. In ‘Advances in Polymer Science’; Publisher: Springer 2015, pp 265-296 (book chapter) PDF_
Bertassoni, LE. Bioprinting human organs. Australasian Science. 2015 April (Invited short-review)
Bertassoni, LE; Swain, MV. The contribution of proteoglycans to the mechanical behavior of dentine and other mineralized tissues. Journal of Mechanical Behavior of Biomedical Materials; 2014;38:91-104 ​
Shahmoradi, M; Bertassoni, LE; Elfallah, H; Swain MV. Fundamental Structure and Properties of Enamel, Dentin and Cementum. In Advances in Calcium Phosphate Biomaterials. (2014) Publisher: Springer. Volume 2, 2014, pp 511-547 (book chapter)
Guazzato, M; Clarke, I; Aquilla, A; Bertassoni, LE; Klineberg, I. (2013) Surface roughness of crown margin preparations: a comparative analysis of finishing techniques. International Journal of Periodontology & Restorative Dentistry. In press ​
Bertassoni, LE; Marshall, GW; Swain, MV. (2012) Mechanical Heterogeneity of Dentin at Different Length Scales as Determined by AFM Phase Contrast. Micron. 2012 Dec;43(12):1364-71 ​
Bertassoni, LE; Orgel, JPR; Antipova, O; Swain, MV. (2012) The Dentin Organic Matrix – Limitations of Restorative Dentistry Hidden on the Nanometer Scale. Acta Biomaterialia. 2012;8:2419-33 ​

Bertassoni LE, Swain MV. Influence of hydration on nanoindentation induced energy expenditure of dentin. Journal of Biomechanics. 2012 1;45:1679-83. 
​
Bertassoni LE, Stankoska K, Sawin MV. Insights into the structure and composition of peritubular dentin organic matrix and the lamina limitans. Micron 2012;43:229-36 ​
Bertassoni LE; Marshall SJ;  Marshall GW, Habelitz S. Mechanical Recovery of Dentin Following Remineralization In Vitro – an Indentation Study. Journal of Biomechanics. 2011;44:176-81 ​
Bertassoni LE; Pugach MK; Soares PC; Marshall SJ;  Marshall GW, Habelitz S. Evaluation of Surface Structural and Mechanical Changes Following Remineralization of Dentin. Scanning 2010;32:312-9 
​
Uskokovic V* and Bertassoni LE*; Nanotechnology in Dental Sciences: Moving towards a Finer Way of Doing Dentistry. Materials 2010, 3:1674-1691 *Equal contribution  _
Machado D, Bertassoni LE, Souza EM, Almeida JB, Rached RN. Effect of additives on the compressive strength and setting time of a Portland cement. Brazilian Oral Research. 2010;24:158-64
Bertassoni LE, Marshall GW. Papain-gel degrades intact nonmineralized type I collagen fibrils. Scanning 2010;31, 1–6. ​
Zaclikevis MV; D´Agulham AC; Bertassoni LE, Machado MA, Lima AA, Grégio AM; Azevedo-Alanis LR. Effects of benzodiazepines and pilocarpine on rat parotid glands: histomorphometric and sialometric study. Medicinal Chemistry. 2009;5:74-8
Bertassoni LE; Habelitz S;  Kinney JH, Marshall SJ, Marshall GW. Biomechanical perspective on the remineralization of dentin. Caries Research. 2009;43:70-7 ​
Bertassoni LE; Marshall GW; Souza EM; Rached RN. Effect of pre- and postpolymerization on flexural strength and elastic modulus of impregnated fiber reinforced denture base acrylic resins. Journal of Prosthetic Dentistry. 2008;100:449-57
Bertassoni LE; Martin JH; Torno V; Vieira S; Rached RN; Mazur R. In-Office Dental Bleaching and Enamel Microabrasion for Fluorosis Treatment. Journal of Clinical and Pediatric Dentistry 2008;32:185

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